colonization rate of group b streptococcus (gbs) in pregnant women using gbs agar medium
Authors
abstract
group b streptococci (gbs) or streptococcus agalactiae are members of the normal flora of the female genital tract. gbs normally colonizes the vagina in many women asymptomatically. during labor this organism may infect the newborn, leading to neonatal sepsis and meningitis. this study aimed to investigate the prevalence of group b streptococcus in pregnant women by a rapid and easy culture method. it seems that in cases in which gbs carriage is not suspected until the time of labor, using such a quick and specific culture method would be valuable. a total of 330 vaginal swabs were collected from women attending delivery room at hedayat hospital, tehran, iran, from april through july 2008. cotton swabs contaminated with vaginal fluid were placed into amies transport medium and transported to the avicenna laboratory daily. vaginal specimens were cultured on selective gbs agar base medium (islam) for isolation and detection of group b streptococcus. the plates were incubated at 35-37°c under anaerobic condition for 24 hours. incubated s.agalactiae developed orange/red pigmented colonies in gbs agar plates. among the 330 women, the results of the culture were positive for gbs in 68 women (20.6%). statistical analyses showed no significant relationship between demographics, reproductive histories and obstetric characteristics of subjects with the test results. solely the antibiotic therapy was associated with gbs colonization. the results are indicating that the relatively high maternal gbs colonization rate in pregnant women warrants a routine screening and prophylactic treatment of the infected women. colonization with group b streptococcus can be identified directly by gbs agar medium and decrease the time to detection of gbs.
similar resources
Evaluation of the new brilliance GBS chromogenic medium for screening of Streptococcus agalactiae vaginal colonization in pregnant women.
Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromID STRB medium (bioMérieux), respectively.
full textMaternal Group B Streptococcal (GBS) Genital Tract Colonization at Term in Women who Have Asymptomatic GBS Bacteriuria
OBJECTIVE To determine the rate of positive group B streptococcus (GBS) cultures at 35-37 weeks gestation in women who have first trimester asymptomatic GBS bacteriuria. METHODS Pregnant women with asymptomatic first trimester GBS bacteriuria had genital cultures for GBS performed at 35-37 weeks gestational age. Serotyping was performed by the standard Lancefield capillary precipitin method. ...
full textThe Prevalence of Group B Streptococcus Colonization in Iranian Pregnant Women and Its Subsequent Outcome
full text
Relationship between Colonization of Group B Streptococcal (GBS) in the Reproductive System of Pregnant Women with PCR with Neonatal and Maternal Complications
Background and Aim: Group B Streptococcus (GBS) is a major contributor to maternal and neonatal death. GBS colonization in the mother can be transient or intermittent, which is why its examination during delivery is more valuable than screening at lower gestational age. The aim of this study was to compare the complications of premature delivery with term ones in positive cases of GBS by PCR me...
full textMulticenter study evaluating performance of the Smart Group B Streptococcus (GBS) assay using an enrichment protocol for detecting GBS colonization in patients in the antepartum period.
Group B streptococcus (GBS) remains the leading cause of infectious morbidity and mortality in infants born in the United States, especially among black infants. Because a newborn can acquire GBS during and after delivery, the Centers for Disease Control and Prevention (CDC) recommends that pregnant women be screened for rectovaginal GBS colonization during the antepartum period between weeks 3...
full textVaginal microbiota in healthy pregnant women and prenatal screening of group B streptococci (GBS).
The microbiota of the lower female genital tract was evaluated from vaginal swabs obtained from 623 healthy pregnant women at gestation periods of 35-40 weeks. Isolated and identified microorganisms were expressed as percentages of total samples. As expected, lactobacilli made up the dominant vaginal microbiota (70%). Enterobacteriaceae, mainly Escherichia coli, Klebsiella spp. and Proteus, wer...
full textMy Resources
Save resource for easier access later
Journal title:
acta medica iranicaجلد ۴۷، شماره ۱، صفحات ۲۵-۳۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023